This page is an archival copy for indexing and accessibility purposes. All figures have been removed to decrease the page size. See https://doi.org/10.1021/acsami.6b16105 for the final version.

Analysis of Scanning Electron Microscopy Images To Investigate Adsorption Processes Responsible for Detection of Cancer Biomarkers

Valquiria Da Cruz Rodrigues, Cesar H. Comin (ORCID), Juliana Coatrini Soares, Andrey Coatrini Soares, Matias Eliseo Melendez, José Humberto T. G. Fregnani, André Lopes Carvalho, Luciano Da F. Costa, Osvaldo N. Oliveira

ACS Applied Materials & Interfaces, 9(7): 5885-5890. . DOI: 10.1021/acsami.6b16105.

Abstract

Adsorption processes are responsible for detection of cancer biomarkers in biosensors (and immunosensors), which can be captured with various principles of detection. In this study, we used a biosensor made with nanostructured films of polypyrrole and p53 antibodies, and image analysis of scanning electron microscopy data made it possible to correlate morphological changes of the biosensor with the concentration of cells containing the cancer biomarker p53. The selectivity of the biosensor was proven by distinguishing images obtained with exposure of the biosensor to cells containing the biomarker from those acquired with cells that did not contain it. Detection was confirmed with cyclic voltammetry measurements, while the adsorption of the p53 biomarker was probed with polarization-modulated infrared reflection absorption (PM-IRRAS) and a quartz crystal microbalance (QCM). Adsorption is described using the Langmuir–Freundlich model, with saturation taking place at a concentration of 100 Ucells/mL. Taken together, our results point to novel ways to detect biomarkers or any type of analyte for which detection is based on adsorption as is the case of the majority of biosensors.

Citation

@article{Rodrigues2017Analysis,
  title   = {Analysis of Scanning Electron Microscopy Images To Investigate Adsorption Processes Responsible for Detection of Cancer Biomarkers},
  author  = {Rodrigues, Valquiria Da Cruz and Comin, Cesar H. and Soares, Juliana Coatrini and Soares, Andrey Coatrini and Melendez, Matias Eliseo and Fregnani, José Humberto T. G. and Carvalho, André Lopes and Costa, Luciano Da F. and Oliveira, Osvaldo N.},
  journal = {ACS Applied Materials & Interfaces},
  year    = {2017},
  volume  = {9},
  number  = {7},
  pages   = {5885--5890},
  doi     = {10.1021/acsami.6b16105}
}